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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential role in advertising cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two extra splice variants were being documented but specific nucleotide sequence facts isn't nevertheless accessible.

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Blocking peptides are peptides that bind specially to your concentrate on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.

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This manual outlines further more possible optimization of mobile and immunoassay parameters to ensure the very best final results are...

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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best results are...

Antibodies bound to the blocking peptide now not bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.

Target Information Cyclin E belongs towards the very conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. click here Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress through S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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